ELISA Analysis, ELISA Data Analysis – What is it?

ELISA Analysis is a broad term that describes the analysis of the data obtained from a ELISA based experiment.  A basic ELISA experiment involves a 96 well microplate with a series of wells used to produce a standard curve from known concentrations, a small number of blank wells and the remainder of the plate used for samples with unknown concentrations.  ELISA Analysis involves the following steps:

  1. Fitting of a model curve to the standard data to produce a Standard Curve
  2. Use the blank wells to identify the level of background noise and exclude data that is undifferentiatable form the background noise
  3. Use the Standard Curve to predict the concentrations of the unknown samples

There are many advanced ELISA Analysis techniques and these will be discussed in specific knowledge base posts.

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