Adjusting to allow for Non-Specific Background (NSB)

ELISA is a robust assay, but it is inevitable that there will be some binding of proteins to the plate that is not intended. Using NSB wells in your plate layout will give you the option to subtract out this background noise from your intensity readings before analysis.

To do this with your should specify the NSB wells in your plate layout in step 2 of the work flow and select the check box “Subtract Non-Specific Binding (NSB) Control well average OD from all other ODs?” in step 3. will do the rest.