ELISA Analysis is a broad term that describes the analysis of the data obtained from a ELISA based experiment. A basic ELISA experiment involves a 96 well microplate with a series of wells used to produce a standard curve from known concentrations, a small number of blank wells and the remainder of the plate used for samples with unknown concentrations. ELISA Analysis involves the following steps:
- Fitting of a model curve to the standard data to produce a Standard Curve
- Use the blank wells to identify the level of background noise and exclude data that is undifferentiatable form the background noise
- Use the Standard Curve to predict the concentrations of the unknown samples
There are many advanced ELISA Analysis techniques and these will be discussed in specific knowledge base posts.