5PL ELISA Curve, 5 Parameter Logistic Curve Fitting

The 5 Parameter Logistic (5PL) curve is recommended by some ELISA kit manufactures for fitting a standard curve.  For those without a statistics degree (most of us in the life sciences) it can sound pretty intimidating.  The good news is that ELISA Software like elisaanalysis.com will do the hard work for you by fitting the curve and predicting your unknown values without you needing to know the details.  But if you want to get a more detailed understanding of the 5PL curve then we suggest that you refer to page 327 of the The Immunoassay Handbook, Forth Edition.  A preview of the relivant pages can be found on Google Books via the following link: http://books.google.com.au/books?id=xuYf6tcVdqYC&lpg=PA329&dq=4pl%20elisa%20curve%20fitting&pg=PA327#v=onepage&q&f=false