Linear ELISA Curve, Linear Curve Fitting

A linear curve can be used for fitting an ELISA standard curve and can provide a reasonable fit for readings in the middle of the assay range.  If you want to get a more detailed understanding of linear curve fitting then we suggest that you refer to page 327 of the The Immunoassay Handbook, Forth Edition.  A preview of the relivant pages can be found on Google Books via the following link: