What does the option “starting reference number” mean in Step 2 when creating a layout?

The “starting reference number” relates to the next sample number the investigator is going to mark on the plate layout. It can either be the standard or the unknowns.  For example, in the plate layout if you have already marked wells as Unknowns 1-3, then the “starting reference number” should be set to 4 for the next Unknown sample (this should automatically happen). The same concept is followed for the standards. Soon we will be offering more powerful labelling in our plate layouts to specify detailed descriptions rather than just number references.