What should I do when I have controls/negatives for two different conditions on the same plate?

This experiment involves comparing pairs of treatment and control replicates on one plate. At present ELISAAnalysis software does not have the option of associating specific control/negative wells with specific treatment wells. Any wells marked as negatives are used to calculate the background cut-off for all unknowns on the plate.

There are two options for working around this limitation:

  1. Split the plate into two smaller data sets and analyse these data sets separately with ELISAAnalysis.com
  2. Specify the controls and treatment wells as “Unknowns” in the plate layout. ELISAAnalysis will calculate the concentrations for both and the investigator can then manually compare the controls vs. treatment group in the results.